Sanger測(cè)序，也稱為鏈終止法，是一種基于DNA聚合酶選擇性摻入鏈終止雙脫氧核苷酸（ddNTPs）的DNA測(cè)序技術(shù)。雖然新的NGS技術(shù)由于其較高的通量能力和較低的每份樣品成本而在臨床研究實(shí)驗(yàn)室中變得很普遍，但Sanger測(cè)序仍具有99.99％的準(zhǔn)確度。四種不同的熒光ddNTP（標(biāo)記為BigDye®，BigDye®是ThermoFisher的商標(biāo)）是執(zhí)行Sanger測(cè)序的關(guān)鍵成分。MagaDye 588-ddTTP等同于BigDye dROX，具有幾乎相同的光譜。百螢生物是AAT Bioquest的中國代理商，為您提供的MagaDye 588-ddTTP。

參考文獻(xiàn)

A novel gross deletion and breakpoint junction sequence analysis of ATP7B in a Chinese family with Wilson disease using next?generation sequencing and Sanger sequencing.
Authors: Liu, Wei-Liang and Li, Fang and Liu, Lu and Chen, Wei and He, Zhi-Xu and Gu, Hao and Ai, Rong
Journal: Molecular medicine reports (2020): 517-523

Concurrent Cultivation of Mycobacterium avium and Mycobacterium intracellulare Identified by a Single Sanger Sequencing of the 16S Gene.
Authors: Han, Xiang Y and Golshan, Mohammad A and Bowman, Christopher J
Journal: Journal of clinical microbiology (2020)

Detection of TERT promoter mutation in serum cell-free DNA using wild-type blocking PCR combined with Sanger sequencing in hepatocellular carcinoma.
Authors: Akuta, Norio and Suzuki, Fumitaka and Kobayashi, Mariko and Fujiyama, Shunichiro and Kawamura, Yusuke and Sezaki, Hitomi and Hosaka, Tetsuya and Kobayashi, Masahiro and Saitoh, Satoshi and Arase, Yasuji and Ikeda, Kenji and Suzuki, Yoshiyuki and Kumada, Hiromitsu
Journal: Journal of medical virology (2020)