碘化丙啶(PI)屬于與溴化乙錠相同的化學(xué)類(lèi)別。與溴化乙錠的情況一樣,在與核酸結(jié)合后的熒光增強(qiáng)了20-30倍。熒光激發(fā)大值紅移為30-40nm,熒光發(fā)射大值藍(lán)移為15nm左右。PI通常用于鑒定細(xì)胞群中的死細(xì)胞,并用作多色熒光技術(shù)中的復(fù)染劑。它還可用于區(qū)分壞死細(xì)胞,凋亡細(xì)胞和正常細(xì)胞。它適用于熒光顯微鏡,流式細(xì)胞儀和熒光儀。
Citations
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Chlorite and bromate alter the conjugative transfer of antibiotic resistance genes: Co-regulation of oxidative stress and energy supply
Authors: Cao, Jinrui and Xue, Bin and Yang, Shuran and Yang, Xiaobo and Zhang, Xi and Qiu, Zhigang and Shen, Zhiqiang and Wang, Jingfeng
Journal: Journal of Hazardous Materials (2024): 134257
Cylindrospermopsin enhances the conjugative transfer of plasmid-mediated multi-antibiotic resistance genes through glutathione biosynthesis inhibition
Authors: Yang, Shuran and Cao, Jinrui and Zhao, Chen and Zhang, Xi and Li, Chenyu and Wang, Shang and Yang, Xiaobo and Qiu, Zhigang and Li, Chao and Wang, Jingfeng and others,
Journal: Ecotoxicology and Environmental Safety (2024): 116288
Galectin-1 overexpression induces normal fibroblasts translate into cancer-associated fibroblasts and attenuates the sensitivity of anlotinib in lung cancer
Authors: Zhang, Lei and Chen, Wenbang and Li, Xiaojun and Wang, Gengming and Xing, Fubao and Zhu, Xiao
Journal: Cell Adhesion \& Migration (2024): 1--11
Microfluidic System for Cell Mixing and Particle Focusing Using Dean Flow Fractionation
Authors: Wiede, Alexander and Stranik, Ondrej and Tannert, Astrid and Neugebauer, Ute
Journal: (2023): 671--685
Development and characterization of biomimetic peptide-based bioink for dental tissue engineering
Authors: Altan, Zeynep
Journal: (2023)