DNA定量是DNA樣品制備過程中的一項(xiàng)重要工作,Helixyte 綠色雙鏈DNA定量檢測試劑盒提供了一種用Helixyte Green BR快速測定dsDNA的方法。該測定法在三個(gè)數(shù)量級(jí)上是線性的,并且比紫外線吸收度讀數(shù)靈敏度高幾個(gè)數(shù)量級(jí)。Helixyte Green-BR與dsDNA結(jié)合后熒光增強(qiáng),序列依賴性小,可準(zhǔn)確測定基因組DNA、病毒DNA、miniprep-DNA或PCR擴(kuò)增產(chǎn)物等多種來源的DNA樣品。該方法對(duì)RNA上的雙鏈DNA(dsDNA)具有高度的選擇性,并且被優(yōu)化以測量從10 pg/ul到10 ng/ul的DNA濃度。百螢生物是AAT Bioquest的中國代理商,為您提供的Helixyte 綠色雙鏈DNA定量檢測試劑盒。
參考文獻(xiàn)
A new reporter design based on DNA origami nanostructures for quantification of short oligonucleotides using microbeads
Authors: Choi, Y., Schmidt, C., Tinnefeld, P., Bald, I., Rodiger, S.
Journal: Sci Rep (2019): 4769
A universal fluorescence-based toolkit for real-time quantification of DNA and RNA nuclease activity
Authors: Sheppard, E. C., Rogers, S., Harmer, N. J., Chahwan, R.
Journal: Sci Rep (2019): 8853
Effects of Quantification Methods, Isolation Kits, Plasma Biobanking, and Hemolysis on Cell-Free DNA Analysis in Plasma
Authors: Streleckiene, G., Forster, M., Inciuraite, R., Lukosevicius, R., Skieceviciene, J.
Journal: Biopreserv Biobank (2019): ersion="1.0" encoding="UTF-8" ?>17645.enlEndNote1117Streleckiene, G.Forster, M.Inciuraite, R.Lukosevicius, R.Skieceviciene, J.1Institute for Digestive Research, Lithuanian University of Health Sciences, Kaunas, Lithuania. 2Institute of Clinical Molecu
Flat-top TIRF illumination boosts DNA-PAINT imaging and quantification
Authors: Stehr, F., Stein, J., Schueder, F., Schwille, P., Jungmann, R.
Journal: Nat Commun (2019): 1268