該試劑盒提供了一種優(yōu)化的測(cè)定方法,用于檢測(cè)G蛋白偶聯(lián)受體(GPCR)和鈣通道。
鈣通量測(cè)定法是藥品發(fā)現(xiàn)中篩選G蛋白偶聯(lián)受體(GPCR)的方法。該試劑盒使用高度鈣敏感和膜通透的腔腸素類似物作為用載脂蛋白基因轉(zhuǎn)染細(xì)胞的鈣指示劑。水母發(fā)光蛋白是來自維多利亞水母的鈣敏感生物發(fā)光蛋白,已廣泛用作細(xì)胞中的鈣指示劑。當(dāng)與鈣離子結(jié)合時(shí),水母發(fā)光蛋白復(fù)合物發(fā)出藍(lán)光。發(fā)光強(qiáng)度與Ca 2+濃度成正比。我們的腔腸素試劑盒比熒光鈣測(cè)定試劑盒(如Fluo-4,F(xiàn)luo-3,Calcium-3和Calcium-4)靈敏得多。該試劑盒提供了一種優(yōu)化的測(cè)定方法,用于檢測(cè)G蛋白偶聯(lián)受體(GPCR)和鈣通道。該測(cè)定可以使用96孔或384孔微孔板進(jìn)行,并易于適應(yīng)自動(dòng)化。百螢生物是AAT Bioquest的中國代理商,為您提供的Screen Quest 發(fā)光法鈣離子檢測(cè)試劑盒。
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