鈣通量分析是篩選G蛋白偶聯(lián)受體（GPCR）藥品發(fā)現(xiàn)的方法。該比值鈣測(cè)定試劑盒允許均勻測(cè)量由G蛋白偶聯(lián)受體或鈣通道引起的細(xì)胞內(nèi)鈣變化。表達(dá)通過鈣傳遞信號(hào)的GPCR的細(xì)胞被Fura-2AM預(yù)加載，F(xiàn)ura-2AM可以穿過細(xì)胞膜。一旦進(jìn)入細(xì)胞內(nèi)，親脂封閉基團(tuán)Fura-2AM被酯酶裂解，導(dǎo)致帶負(fù)電荷的熒光染料留在細(xì)胞內(nèi)，其熒光波長(zhǎng)在與鈣結(jié)合時(shí)藍(lán)移。當(dāng)細(xì)胞受到激動(dòng)劑刺激時(shí)，受體發(fā)出細(xì)胞內(nèi)鈣離子釋放的信號(hào)，從而大大提高Fura-2在短波處的熒光強(qiáng)度。Fura-2的比色特性使該試劑盒成為比單波長(zhǎng)Fluo-4測(cè)定細(xì)胞鈣濃度的理想工具。通過單次添加，該分析很容易在高透性環(huán)境中進(jìn)行。該方法可用于96孔或384孔微孔板，且易于自動(dòng)化。百螢生物是AAT Bioquest的中國(guó)代理商，為您提供的Screen Quest 免洗Fura-2鈣檢測(cè)試劑盒

參考文獻(xiàn)

Calcium imaging of cortical neurons using Fura-2 AM
Authors: Barreto-Chang OL, Dolmetsch RE.
Journal: J Vis Exp. (2009)

Measurement of [Ca2+]i in whole cell suspensions using fura-2
Authors: Hirst RA, Harrison C, Hirota K, Lambert DG.
Journal: Methods Mol Biol (2006): 37

Load of calcium probe Fura -2/AM in Escherichia coli cells
Authors: Shao M, Wang HM, Liu ZH, Shen P, Cai RX.
Journal: Wei Sheng Wu Xue Bao (2005): 805

Abnormal spectra alteration observed in Triton calibration method for measuring [Ca2+]i with fluorescence indicator, fura-2
Authors: Xu T, Yang W, Huo XL, Song T.
Journal: J Biochem Biophys Methods (2004): 219

Fluorescence measurements of free [Mg2+] by use of mag-fura 2 in Salmonella enterica
Authors: Froschauer EM, Kolisek M, Dieterich F, Schweigel M, Schweyen RJ.
Journal: FEMS Microbiol Lett (2004): 49