Screen Quest 比色法葡萄糖攝取檢測(cè)試劑盒是美國AAT Bioquest生產(chǎn)的用于葡萄糖檢測(cè)的試劑盒,葡萄糖轉(zhuǎn)運(yùn)系統(tǒng)負(fù)責(zé)將葡萄糖轉(zhuǎn)運(yùn)穿過細(xì)胞膜。
Screen Quest 比色法葡萄糖攝取檢測(cè)試劑盒是美國AAT Bioquest生產(chǎn)的用于葡萄糖檢測(cè)的試劑盒,葡萄糖轉(zhuǎn)運(yùn)系統(tǒng)負(fù)責(zé)將葡萄糖轉(zhuǎn)運(yùn)穿過細(xì)胞膜。測(cè)量組織和細(xì)胞中2-葡萄糖類似物2-脫氧葡萄糖(2-DG)的攝取被廣泛接受作為估計(jì)葡萄糖攝取量和研究葡萄糖代謝的調(diào)節(jié)和胰島素抗性機(jī)制的可靠方法。 2-DG攝取通常通過使用用氚或C14標(biāo)記的非代謝的2-DG來確定。然而,常規(guī)使用放射性標(biāo)記的探針是昂貴的并且需要繁瑣的特殊處理程序。 AAT Bioquest的Screen Quest?比色葡萄糖攝取分析試劑盒可在組織或培養(yǎng)細(xì)胞中提供靈敏的非放射性分析。在該測(cè)定中,2-DG被葡萄糖轉(zhuǎn)運(yùn)蛋白吸收,并代謝成2-DG-6-磷酸(2-DG6P)。不可代謝的2-DG6P在細(xì)胞中積累,并且與細(xì)胞的葡萄糖攝取成比例。累積的2-DG6P被酶促氧化并產(chǎn)生NADPH,其通過顯色NADPH特異性檢測(cè)。通過讀取波長(zhǎng)570nm至610nm的OD比,可以通過吸收酶標(biāo)儀讀取信號(hào)。百螢生物是AAT Bioquest的中國代理商,為您提供的Screen Quest 比色法葡萄糖攝取檢測(cè)試劑盒。
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