膽堿及其代謝產(chǎn)物在細(xì)胞膜的結(jié)構(gòu)完整性和信號傳導(dǎo)以及膽堿能神經(jīng)傳遞（乙酰膽堿合成）中起著重要作用。它是通過其代謝產(chǎn)物基甘氨酸參與S-腺苷甲硫氨酸合成途徑而獲得甲基的主要來源。膽堿缺乏癥可能導(dǎo)致肝臟病癥，動脈粥樣硬化以及可能的神經(jīng)系統(tǒng)病癥。盡管膽堿在神經(jīng)系統(tǒng)中作為乙酰膽堿和膜磷脂酰膽堿的前體具有重要作用，但膽堿在精神病中的作用尚未得到研究。該Amplite 膽堿定量試劑盒提供了靈敏的膽堿定量方法之一。該試劑盒使用Amplite Red量化膽堿的濃度，這與膽堿氧化酶介導(dǎo)的酶偶聯(lián)反應(yīng)中過氧化氫的產(chǎn)生有關(guān)。膽堿的量與在酶偶聯(lián)反應(yīng)循環(huán)中形成的過氧化氫的濃度成比例。在存在過氧化物酶的情況下，Amplite Red的熒光強(qiáng)度與過氧化氫的形成成比例，該過氧化氫轉(zhuǎn)化為膽堿的濃度。該測定可以容易地用熒光酶標(biāo)儀讀取，或者也可以使用光吸收酶標(biāo)儀在?570 nm處讀取測定值。百螢生物是AAT Bioquest的中國代理商，為您提供的Amplite 膽堿定量試劑盒。

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